Appointment:
- 80% Research, 20% Teaching
Education:
- B.S. in Biology, Lock Haven University, 1981
- M.S. in Entomology, University of Florida, 1984
- Ph.D. in Entomology, Pennsylvania State University, 1989
Professional Responsibilities and Activities:
My research program is involved with a number of projects to determine the biochemical and genetic mechanisms of insecticide resistance in insect pest species. Identification of metabolic pathways, target sites and the molecular basis of resistance are essential to the development of rational control strategies that minimize the adverse effects of resistance development and insecticide use. Additionally, my lab is involved with development of resistance monitoring programs for detection of resistant populations and documenting the distribution of such populations.
Another aspect of my program involves research to identify the physiological and biochemical basis of selective pesticide toxicity in non-target aquatic organisms. This research attempts to develop an understanding of the factors responsible for selective toxicity among aquatic organisms including both invertebrates and freshwater algae. Such information is critical to understanding the ecological consequences of pesticide contamination of surface water.
Courses Taught:
- Insecticide Toxicology (Entomology 820): A study of the principles of toxicology as they relate to insecticides and insect pest species. Emphasis on insecticide classification, mode of action, metabolism and environmental consequences of insecticide use. This course is also delivered via distance education.
- Toxins in the Environment (Entomology 300): An introduction to the principles of toxicology as they apply to environmental contaminants, emphasizing agrochemicals, but also including industrial and naturally occurring chemicals.
Publication Summary:
103 refereed journal articles; 6 reviews/book chapters; 14 refereed abstracts; 12 technical reports, and 6 extension articles.
Recent Publications:
- Magalhaes, L.C., T.E. Hunt, and B.D. Siegfried. 2009. Efficacy of Neonicotinoid seed treatments to reduce soybean aphid populations under field and controlled conditions in Nebraska. J. Econ. Entomol. (In press).
- Prasifka, J.R., R.L. Hellmich, D.V. Sumerford, and B.D. Siegfried. 2009. Bacillus thuringiensis resistance alleles influence European corn borer (Lepidoptera: Crambidae) larval behavior following exposure to Cry1Ab. J. Econ. Entomol. (In press).
- Crespo, A.L.B., T. Spencer, A.P. Alves, R.L. Hellmich, E.E. Blankenship, L.C. Magalhaes, and B.D. Siegfried. 2009. On-plant survival and inheritance of resistance to Cry1Ab toxin from Bacillus thuringiensis in a field-derived strain of European corn borer, Ostrinia nubilalis. Pest Manag. Sci. (In press).
- Bel, Y., H.A.A. Siqueira, B.D. Siegfried, J. Ferre, and B. Escriche. 2009. Variability in the cadherin gene in Ostrinia nubilalis selected for Cry1Ab resistance. Insect Molec. Biol. (In press).
- Coates, B.S., D.V. Sumerford, K.S. Kim, N.J. Miller, T.W. Sappington, B.D. Siegfried, and L.C. Lewis. 2009. Comparative performance of single nucleotide polymorphisms (SNP) and microsatellite markers for population genetic analysis. Heredity. (In press).
- Crespo, A.L.B., T.A. Spencer, E. Nekl, and B.D. Siegfried. 2008. Comparison and validation of methods to quantify Cry1Ab from Bacillus thuringiensis for standardization of insect bioassays. Appl. Environ. Microbiol. 74: 130-135.
- Alves, A.P., W.J. Allgeier, and B.D. Siegfried. 2008. Effects of the synergist S,S,S - tributyl phosphorotrithioate on indoxacarb toxicity and metabolism in the European corn borer, Ostrinia nubilalis (Hübner). Pestic. Biochem. Physiol 90: 26-30.
- Blanco, C.A., N.P. Storer, C.A. Abel, R. Jackson, R. Leonard, J.D. Lopez, G. Payne, B.D. Siegfried, T. Spencer, and A.P. Teran-Vargas. 2008. Baseline susceptibility of tobacco budworm (Lepidoptera: Noctuidae) to Cry1F toxin from Bacillus thuringiensis. J. Econ. Entomol. 101: 168-173.
- Pereira, E.J.G., B.A. Lang, N.P. Storer, and B.D. Siegfried. 2008. Selection for Cry1F resistance in the European corn borer and cross resistance to other Cry toxins. Entomol. Exper. Appl. 126: 115-121.
- Pereira, E.J.G., N.P. Storer, and B.D. Siegfried. 2008. Inheritance of Cry1F resistance in laboratory-selected European corn borer and its survival on transgenic corn expressing the Cry1F toxin. Bull. Entomol. Research 98: 621-629.
- Nowatzki, T.M., S.A. Lefko, R.R. Binning, S.D. Thompson, T.A. Spencer, and B.D. Siegfried. 2008. Validation of a novel resistance monitoring technique for corn rootworm (Coleoptera: Chrysomelidae) and Event DAS-59122-7 maize. J. Appl. Entomol. 132: 177-188.
- Magalhaes, L.C., B.W. French, T.E. Hunt, and B.D. Siegfried. 2008. Development of methods to evaluate susceptibility of soybean aphid to imidacloprid and thiamethoxam at lethal and sublethal concentrations. Entomol. Exp. Appl. 128: 330-336.
- Meihls, L.N., M. L. Higdon, B.D. Siegfried, N.J. Miller, T.W. Sappington, M.R. Ellersieck, T.A. Spencer, and B.E. Hibbard. 2008. Rapid evolution of resistance to Bt corn by western corn rootworm: evidence for multiple resistance mechanisms? Proc. Nat. Acad. Sci. 49: 19177-19182.
- Ciosi, M., S. Toepfer, H. Li, T. Haye, U. Kuhlmann, H. Wang, B. Siegfried, and T. Guillemaud. 2008. Are the invading Western corn rootworm (Coleoptera: Chrysomelidae) populations in Europe resistant against insecticides as in the USA? J. Entomol. Sci. (In press).
- Magalhaes, L.C., B.W. French, T.E. Hunt, and B.D. Siegfried. 2007. Baseline susceptibility of western corn rootworm (Coleoptera: Chrysomelidae) to clothianidin. J. Appl. Entomol. 131: 251-255.
- Londoño, D.K., H.A.A. Siqueira, H. Wang, G. Sarath, M.J. Lydy, and B.D. Siegfried. 2007. Cloning and expression of an atrazine inducible cytochrome P450, Cyp4G33, from Chironomus tentans (Diptera: Chironomidae). Pestic. Biochem. Physiol. 89: 104-110.
- Sayed, A., E.R. Nekl, H.A.A. Siqueira, H. Wang, R.H. ffrench-Constant, M. Bagley, and B.D. Siegfried. 2007. A novel cadherin-like gene from western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae), larval midgut tissue. Insect Biochem. Molec. Biol. 16: 591-600.
- Siegfried, B.D., T. Spencer, T., A.L. Crespo, N.P. Storer, G.P. Head, E.D. Owens, and D. Guyer. 2007. Ten years of monitoring for Bt resistance in the European corn borer: What we know, what we don't know and what we can do better. Amer. Entomol. 53: 208-214.
- Parimi, S., L.J. Meinke, B.W. French, L.D. Chandler, and B.D. Siegfried. 2006. Stability and persistence of aldrin and methyl-parathion resistance in western corn rootworm populations (Coleoptera: Chrysomelidae). Crop Protection 25: 269-274.
- Alves, A.P., T.A. Spencer, B.E. Tabashnik, and B.D. Siegfried. 2006. Inheritance of resistance to the Cry1Ab Bacillus thuringiensis toxin in Ostrinia nubilalis (Lepidoptera: Crambidae). J. Econ. Entomol. 99: 494-501.
- Caprio, M.A., T. Nowatzki, B. Siegfried, L.J. Meinke, R.J. Wright, and L.D. Chandler. 2006. Assessing risk of resistance to aerial applications of methyl-parathion in western corn rootworm (Coleoptera: Chrysomelidae). J. Econ. Entomol. 99: 483-493.
- Lockert, C.K., K.D. Hoagland, and B.D. Siegfried. 2006. Comparative sensitivity of freshwater algae to atrazine. Bull. Environ. Contam. Toxicol. 76: 73-79.
- Nowatzki, T.M., X. Zhou, L.J. Meinke, T. Vaughn, and B.D. Siegfried. 2006. Effect of Bacillus thuringiensis Cry3Bb1 protein on the feeding behavior and longevity of adult western corn rootworms (Coleoptera: Chrysomelidae). J. Econ. Entomol. 99: 927-930.
- Siqueira, H.A.A., J. González-Cabrera, J. Ferré, R. Flannagan, and B.D. Siegfried. 2006. Analyses of Cry1Ab binding in resistant and susceptible strains of the European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae). Appl. Environ. Microbiol. 72: 5318-5324.
- Aliano, N.P., M.D. Ellis, and B.D. Siegfried. 2006. Acute contact toxicity of oxalic acid to Varroa destructor (Acari: Varroidae) and their Apis mellifera (Hymenoptera: Apidae) hosts in laboratory bioassays. J. Econ. Entomol. 99: 1579-1582.
- Sappington, T.W., B.D. Siegfried, and T. Guillemaud. 2006. Coordinated Diabrotica genetics research: Accelerating progress on an urgent insect pest problem. Amer. Entomol. 52: 90-97.
- Le Goff, G., F. Hilliou, B.D. Siegfried, S. Boundy, E. Wajnberg, L. Sofer, P. Audant, R.H. ffrench-Constant, and R. Feyereisen. 2006. Xenobiotic response in Drosophila melanogaster: Sex dependence of P450 and GST gene induction. Insect Biochem. Mol. Biol. 36: 674-682.
- Parimi, S., L.J. Meinke, B.W. French, L.D. Chandler, and B.D. Siegfried. 2006. Persistence and stability of methyl-parathion and aldrin resistance in western corn rootworms. Crop Protection. 25:269-274.
- Alves, A.P., T. Spencer, B.E. Tabashnik, and B.D. Siegfried. 2006. Inheritance of resistance to the Cry1Ab Bacillus thuringiensis toxin in Ostrinia nubilalis (Lepidoptera: Crambidae). J. Econ. Entomol. 99: 494-501.
- Caprio, M.J., T.J. Nowatzki, B.D. Siegfried, L.J. Meinke, R.J. Wright, and L.D. Chandler. 2006. Assessing the risk of resistance to aerial applications of methyl-Parathion in the western corn rootworm (Coleoptera: Chrysomelidae). J. Econ. Entomol. 99: 483-493.
- Lockert, C.K., K.D. Hoagland, and B.D. Siegfried. 2006. Atrazine sensitivity among selected freshwater algae. Bull. Environ. Contamin. Toxicol. 761: 73-79.
- Nowatzki, T.M., X. Zhou, L.J. Meinke, T.T. Vaughn, and B.D. Siegfried. 2006. Effect of Bacillus thuringiensis Cry3Bb1 protein on the feeding behavior and longevity of adult western corn rootworms (Coleoptera: Chrysomelidae). J. Econ. Entomol. 99: 927-930.
- Siqueira, H.A.A., J. Gonzalez-Cabrera, J. Ferre, R. Flannagan, and B.D. Siegfried. 2006. Cry1Ab binding analyses in resistant and susceptible strains of European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae). Appl. Environ. Microbiol. 72: 5318-5324.
- Aliano, N.P., M.D. Ellis, and B.D. Siegfried. 2006. Acute contact toxicity of oxalic acid to Varroa destructor (Acari: Varroidae) and their Apis mellifera (Hymenoptera: Apidae) hosts in laboratory bioassays. J. Econ. Entomol. 5: 1578-1582.
- Sappington, T.W., B.D. Siegfried, and T. Guillemaud. 2006. Coordinated Diabrotica genetics research: accelerating progress on an urgent insect pest problem. Amer. Entomol. 52: 92-99.
- Le Goff, G., F. Hilliou, B.D. Siegfried, S. Boundy, E. Wajnberg, L. Sofer, P. Audant, R. H. ffrench-Constant, and R. Feyereisen. 2006. Xenobiotic response in Drosophila melanogaster: Sex dependence of P450 and GST gene induction. Insect Biochem. Mol. Biol. 36: 674-682.
- Zhou, X., M.E. Scharf, L.J. Meinke, L.D. Chandler, and B.D. Siegfried. 2005. Immunological assessment of an insecticide resistance-associated esterase in the western corn rootworm. Arch. Insect Biochem. Physiol. 58:157-165.
- Siegfried, B.D., N. Waterfield, and R.H. ffrench-Constant. 2005. Expressed sequence tags from Diabrotica virgifera virgifera midgut identify a coleopteran cadherin and a diversity of cathepsins. J. Insect Molecular Biology. (In press)
- Siegfried, B.D., T. Spencer, and T.T. Vaughn. 2005. Baseline susceptibility of western corn rootworm (Coleoptera:Chrysomelidae) to the Cry3Bb1 Bacillus thuringiensis Toxin. J. Econ. Entomol. 98:1320-1324.
- Flannagan, R.D., C.-G. Yua, J.P. Mathis, T.E. Meyers, X. Shia, H.A.A. Siqueira, and B.D. Siegfried. 2005. Identification, cloning and expression of a Cry1Ab receptor from European corn borer, Ostrinia nubilalis, (Hübner) (Lepidoptera: Crambidae). Insect Biochem. Mol. Biol. 35: 33-40.
- Siegfried, B.D., L.J. Meinke, S. Parimi, M.E. Scharf, T.J. Nowatzki, X. Zhou, and L.D. Chandler. 2004. Monitoring western corn rootworm (Coleoptera: Chrysomelidae) susceptibility to carbaryl and cucurbitacin baits in the area-wide management pilot program. J. Econ. Entomol. 97: 1726-1733.
- Siqueira, H.A.A., K.W. Nickerson, D. Moellenbeck, and B.D. Siegfried. 2004. Activity of gut proteinases from Cry1Ab-selected colonies of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). J. Pest Manag. Sci. 90: 1189-1196.
- Zhou, X., M.E. Scharf, G. Sarath, L.J. Meinke, L.D. Chandler, and B.D. Siegfried. 2004. Partial purification and characterization of a methyl-parathion resistance-associated general esterase in Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae). Pestic. Biochem. Physiol. 78:114-125.
- Siqueira, H.A.A., D. Moellenbeck, T. Spencer, and B.D. Siegfried. 2004. Cross-resistance of Cry1Ab-selected Ostrinia nubilalis (Lepidoptera: Crambidae) to Bacillus thuringiensis Δ-endotoxins. J. Econ. Entomol. 97: 1049-1057.
- Londoño, D.K., M.J. Lydy, and B.D. Siegfried. 2004. Atrazine induction of a family 4 cytochrome P450 gene in Chironomus tentans (Diptera: Chironomidae). Chemosphere 56/57: 701-706.
- Zoerb, A.C., T. Spencer, R.L. Hellmich, R.J. Wright, and B.D. Siegfried. 2003. Larval distribution and survival of second generation European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) on Event 176 Bt corn. Crop Protection 22: 179-184.
- Zhou, X., M.E. Scharf, L.J. Meinke, L.D. Chandler, and B.D. Siegfried. 2003. Characterization of general esterases from methyl parathion-resistant and -susceptible populations of western corn rootworm (Coleoptera: Chrysomelidae). J. Econ. Entomol. 96: 1855-1863.
- Parimi, S., M.E. Scharf, L.J. Meinke, L.D. Chandler, and B.D. Siegfried. 2003. Inheritance of methyl-parathion resistance in Nebraska western corn rootworm populations (Coleoptera: Chrysomelidae). J. Econ. Entomol. 96: 131-136.
- Parimi, S., L.J. Meinke, T. Nowatzki, L.D. Chandler, B.W. French, and B.D. Siegfried. 2003. Toxicity of insecticide-bait mixtures to insecticide resistant and susceptible western corn rootworms (Coleoptera: Chrysomelidae). Crop Protection 22: 781-786.
- Nowatzki, T.M. , B. Niimi, K.J. Warren, S. Putnam, L.J. Meinke, D.C. Gosselin, F.E. Harvey, T.E. Hunt, and B.D. Siegfried. 2003. In-field labeling of western corn rootworm adults (Coleoptera: Chrysomelidae) with rubidium. J. Econ. Entomol. 96: 1750-1759.
- Marçon, P.C.R.R., G.D. Thomas, B.D. Siegfried, J.B. Campbell, and S.R. Skoda. 2003. Resistance status of house flies (Diptera: Muscidae) from southeastern Nebraska beef cattle feedlots to selected insecticides. J. Econ. Entomol. 96: 1016-1020.
- Zhou, X., M.E. Scharf, S. Parimi, L.J. Meinke, R.J. Wright, L.D. Chandler, and B.D. Siegfried. 2002. Diagnostic assays based on esterase-mediated resistance mechanisms in western corn rootworms (Coleoptera: Chrysomelidae). J. Econ. Entomol. 95: 1261-1266.
- Macedo, P.A., M.D. Ellis, and B.D. Siegfried. 2002. Detection and quantification of fluvalinate resistance in Varroa mites in Nebraska. Am. Bee J. July 2002: 523-526.
- Durham, E.W., B.D. Siegfried, and M.E. Scharf. 2002. In vivo and in vitro metabolism of fipronil by larvae of the European corn borer Ostrinia nubilalis. Pest Manag. Sci. 58: 799-804.
Notable Distinctions:
- Research Award of Merit, University of Nebraska Chapter of Gamma Sigma Delta, 2006
- Outstanding Scientist Award, Sigma Xi Nebraska Chapter, 2003
- Recipient of British Biology and Biotechnology Sciences Research Council (BBSRC) Underwood Fund to support international collaboration with BBSRC funded scientists, 2003
- American Distance Education Consortium, Education Program Award (Presented to the Entomology Department), 2002
- USDA-ARS Team Award, National Areawide Integrated Pest Management Program for Corn Rootworm Across the U.S. Corn Belt, 2000
- U.S. Environmental Protection Agency. Food Quality Protection Act, Scientific Review Board. Bacillus thuringiensis (Bt) Plant - Pesticide; Resistance Management and Effects Non-target Organisms, 2000
- U.S. Environmental Protection Agency. FIFRA Scientific Advisory panel, Subpanel on Bacillus thuringiensis (Bt) Plant -Pesticides and Resistance Management, 1998.
- International Life Sciences (ILSI). Expert Panel Evaluation of Insect Resistance Management in Bt Field Corn: A Science-Based Framework for Risk Assessment and Risk Management, 1997 - 1998
- Outstanding Young Scientist Award, University of Nebraska Chapter of Sigma Xi 1994
- University of Nebraska Institute of Agriculture and Natural Resources, Agricultural Research Division, Junior Faculty Recognition for Excellence in Research, 1993
- Selected as foreign exchange student, Kelvin Grove College of Advanced Education, Brisbane, Australia and Lock Haven University, 1981
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