Andre Luiz Barreto Crespo (Ph.D. Candidate)

                     
202 Plant Industry Building Phone: (402) 472 8700
University of Nebraska, E-mail:  crespo@unlserve.unl.edu
Lincoln, 68583-0816 

Education

Graduation Date: February 2008
      

Ph.D. in Entomology 2004                            

University of Nebraska-Lincoln

Dissertation project – Characterization of Resistance to Cry1Ab toxin from Bacillus thuringiensis in the European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae)

MSc in Entomology 2001-2003

Federal University of Viçosa

Dissertation project – Sampling unit for estimating populations of the Tomato fruit borer, Neoleucinodes elegantalis (Lepidoptera: Crambidae), on tomato

BS in Agronomy 1996-2001

Federal University of Viçosa

Publication List

Refereed Journal Articles

1. Pereira, E. J. G., Picanço, M. C., Bacci, L., Crespo, A. L. B., Guedes, R. N. C. . Seasonal mortality factors of the coffee leaf miner Leucoptera coffeella. Bulletin of Entomological Research, v. 97, p.421-432,  2007.

2. Bacci, L., Crespo, A. L. B., Galvan, T. L., Pereira, E. J. G., Picanço, M. C., Silva, G. A., Chediak, M. Toxicity of insecticides to the sweetpotato whitefly (Hemiptera:Aleyrodidae) and its natural enemies. Pest Management Science, v. 63, p. 699-706,  2007.

3. Bacci, L., Picanço, M. C., Gonring, A. H., Guedes, R. N. C., Crespo, A. L. B. .Critical yield components and key loss factors of tropical cucumber crops. Crop Protection, v. 25, p. 1117-1125,  2006.

4. Bacci, L., Fernandes, F. L., Picanço, M. C., Crespo, A. L. B. Seletividade Fisiológica de Inseticidas a Vespas Predadoras (Hymenoptera:Vespidae) de Leucoptera coffeella (Lepidoptera: Lyonetiidae). Bioassay, v. 10, p. 1-7,  2006.

5. Picanço, M.C., Crespo, A.L.B., Ecole, C.C., Badji, C.A., Costa, H., Couto, F.A.D. Damage, decision making, and control of Pseudophilothrips sp. (Thysanoptera:Phlaeothripidae) and its relationship with fungal lesions in guava fruits. Acta Scientiarum, Maringa, v. 25, n. 1, p. 223-230, 2003. 

6. Crespo, A.L.B., Picanço, M. C., Bacci, L., Pereira, E. J. G., Gonring, A. H. Seletividade Fisiológica de inseticidas a Vespidae predadores de Ascia monuste orseis. Pesquisa Agropecuária Brasileira, Brasília, v. 37, n. 3, p. 237-242, 2002. 

7. Galvan, T. L., Picanço, M. C., Bacci, L., Pereira, E. J. G., Crespo, A. L. B. Seletividade de oito inseticidas a predadores de lagartas em citros. Pesquisa Agropecuária Brasileira, Brasília, v. 37, n. 2, p. 117-122, 2002. 

8. Bacci, L., Picanço, M. C., Gusmão, M. R., Crespo, A. L. B., Pereira, E. J. G. Seletividade de Inseticidas a Brevicoryne brassicae (L.) (Hemiptera: Aphididae) e ao Predador Doru luteipes (Scudder) (Dermaptera:Forficulidae). Neotropical Entomology, Londrina, v. 30, n. 4, p. 707-713, 2001. 

Refereed Journal Articles Recently Accepted for Publication

1. Siegfried, B. D., Spencer, T. A., Crespo, A. L. B., Storer N. P., Head, G.P. Ten years of Bt resistance monitoring in the European corn borer: what we know, what we don't know, and what we can do better. American Entomologist, 2007 (In press).

2. Picanço, M. C., Bacci, L., Crespo, A. L. B., Miranda, M. M. M., Martins, J. C. Effect of Integrated Pest Management Practices on Tomato Lycopersicon esculentum, Production and Preservation of Natural Enemies of Pests. Agricultural and Forest Entomology, 2007 (In press).

Refereed Publications in Progress

1. Bacci, L., Pereira, E. J. G., Crespo, A. L. B., Picanço, M. C., Coutinho, D. C. Sena. Eficiência e seletividade de inseticidas para o manejo de mosca branca e inimigos naturais em melancia. Ceres, 2007 (Submitted).

2. Crespo, A. L. B., Spencer, T. A., Nekl, E. R., William, J.M., Siegfried, B. D. Comparison and validation of methods to quantify Cry1Ab toxin from Bacillus thuringiensis for standardization of insect bioassays. Applied and Environmental Microbiology, 2007 (Submitted).

3. Bacci, L., Pereira, E. J.  G., Crespo, A. L. B., Picanço, M. C. Concentration-mortality responses of Myzus persicae and natural enemies to selected insecticides. Pest Management Science, 2008 (In preparation).

4. Crespo, A. L. B., Picanço, M. C., Bacci, L., Guedes, R. N. C., Pereira, E. J. G., Gontijo, L. M. Sampling unit for estimating populations of the Tomato fruit borer, Neolecinodes elegantalis (Lepidoptera: Crambidae), on tomato. Journal of Applied Entomology, 2008 (In preparation).

5. Crespo, A. L. B., Spencer, T. A., Hellmich, R. L., Siegfried, B. D. Inheritance of resistance to Cry1Ab toxin from Bacillus thuringiensis in a field derived strain of Ostrinia nubilalis. Bulletin of Entomological Research, 2008 (In preparation).

6. Crespo, A. L. B., Spencer, T. A., Galvan, T. L., Siegfried, B. D. Fitness costs of resistance to Cry1Ab toxin from Bacillus thuringiensis in a field derived strain of Ostrinia nubilalis. Entomologia Experimentalis et Applicata , 2008 (In preparation).

Book Chapter andConference Papers

1. Siegfried, B. D., Spencer, T., Crespo, A., Pereira, E., Marçon, P. Ten years of Bt resistance monitoring in the European corn borer: what we know, what we don't know, and what we can do better. In: The 9th International Symposium on the Biosafety of Genetically Modified Organisms, Jeju Island, Korea: biosafety research and environmental risk assessment, 2006, (Conference Paper) pp. 168-171.

2. Picanço, M.C., Pereira, E.J.G., Crespo, A.L.B., Semeão, A.A., Bacci, L. Manejo integrado das pragas das fruteiras tropicais. In: Laércio Zambolim. (Org.). Manejo integrado: Fruteiras tropicais - doenças e pragas. 1 ed. Viçosa, MG: UFV, 2002, (Book chapter) p. 513-578.

Honors

-    Rewarded for academic performance, 2007 (Widaman Trust Distinguished Graduate Assistant Award, U$ 2000, Institute of Agriculture and Natural Resources).

-    Winner of Student Oral Presentation Competition, 2007 (Entomological Society of America, North Central Branch Meeting).

-    Runner-up of North Central Branch Entomological Society of America Linnaean Games, 2007.

-    Winner of the National Entomological Society of America Linnaean Games, 2006.

-    Rewarded for academic and scholastic achievement, 2004 (Mary and Charles C. Cooper/Emma I. Sharpless Fellowship U$ 5000.00, Institute of Agriculture and Natural Resources).

-   Rewarded for outstanding academic performance, 2001 (University of Viçosa-Brazil, Institute of Agricultural Sciences, Recognition for the Excellent Academic Performance as Undergraduate Student).

Research

-   Biochemical, Genetic, and Fitness studies to characterize insect resistance to Bacillus thuringiensis Cry1Ab toxin

-   Evaluation of insect resistance to transgenic corn expressing Cry1Ab

-   Standardization of bioassays with Bacillus thuringiensis Cry1Ab toxin from independent batches

-    Insecticide baseline susceptibility studies and monitoring to identify changes in insect susceptibility to insecticides

-    Life table studies for identification of critical factors affecting plant yield

-    Life table studies for identification of critical factors affecting population dynamics of insect pests

-    Conservation of natural enemies of insect pests through use of selective insecticides

-    Ecological field studies to develop sampling programs to manage insect pests

Technical Skills

-   Production and purification of proteins (Bacillus thuringiensis toxins)

-   Quantification of Bacillus thuringiensis toxins using ELISA and SDS - PAGE

-   Western Blotting and Ligand Blotting analysis

-   In vitro enzyme assays

-   Surface Plasmon Resonance (SPR) for study binding of Cry toxins to insect gut brush-border membrane vesicles

-   Execution of insecticide field trials

-   Development of bioassays to evaluate insecticide toxicity against pests and natural enemies

-   Insect rearing: Hemiptera: Aleyrodidae: Bemisia tabaci, Aphididae:Brevicoryne brassicae, Myzus persicae and Macrosyphum euphorbiae; Hymenoptera: Eulophidae: Oomyzus sp., Aphelinidae: Encarsia Formosa, Braconidae: Diaeretiella rapae; Lepidoptera: Gelechidae: Tuta absoluta, Lyonetiidae: Leucoptera coffeella, Crambidae: Ostrinia nubillalis, Pieridae: Ascia monuste orseis, Plutellidae: Plutella xylostella, and Noctuidae: Helicoverpa zea

Participation on Insecticide field Trials

1. Fernandes, F. L. ; Picanço, Marcelo Coutinho ; Crespo, A. L. B. ; Gontijo, Lessando Moreira ; Simeão, A. A. Effects of Actara 250 WG, Calypso and Provado 200 SC on beetles, collembolans, and psocopterans. In: XI Simpósio de Iniciação Científica, 2002, Viçosa, Brazil UFV, 2002. p. 203-203.

2. Fernandes, F. L. ; Picanço, Marcelo Coutinho ; Galvan, T. L. ; Bacci, L. ; Crespo, A. L. B. Efficacy of thiacloprid, cyflutrin, and chlorpirifos for thrips control on lettuce. In: 41st  Congresso Brasileiro De Olericultura, 2001, Brasília. Poster Presentation: Horticultura Brasileira, 2001. v. 19. p. 252-252. 

3. Crespo, A. L. B. ; Picanço, Marcelo Coutinho ; Gontijo, Lessando Moreira ; Simeão, A. A. ; Fernandes, F. L. Effect of three insecticides on detritivorous insects on tomato. In: 41st Congresso Brasileiro De Olericultura, 2001, Brasília. Poster Presentation: Horticultura Brasileira, 2001. v. 19. p. 250-250.

4.  Silva, Ézio Marques da ; Picanço, Marcelo Coutinho ; Berger, Paulo ; Crespo, A. L. B. ; Antônio, Adilson de Castro. Effect of aldicarb on spider populations in cotton. In: 3rd Congresso Brasileiro De Algodão, 2001, Campo Grande. Poster Presentation: 2001. p. 336-338.

5. Fernandes, F. L. ; Picanço, M. ; Galvan, T. L. ; Crespo, A. L. B. ; Bacci, L. Efficacy of insecticides for Caliothrips brasiliensis e Thrips spp. control on lettuce. In: X Simpósio de Iniciação Científica, 2000, Viçosa, Brazil UFV, 2000. p. 230-230.

6. Gontijo, Lessando Moreira ; Picanço, M. ; Galvan, T. L. ; Crespo, A. L. B. ; Simeão, A. A. Efficacy of insecticides for leaf-miner control on cucumber. In: X Simpósio de Iniciação Científica, 2000, Viçosa, Brazil UFV, 2000. p. 229-229.

7. Campos, S. C. ; Picanço, M. ; Pereira, E. J. G. ; Bacci, L. ; Crespo, A. L. B. Efficacy of insecticides for aphid and whitefly control on beans. In: X Simpósio de Iniciação Científica, 2000, Viçosa, Brazil UFV, 2000. p. 226-226.

8. Coelho, T. G. ; Picanço, M. ; Simeão, A. A. ; Crespo, A. L. B. ; Gontijo, Lessando Moreira. Effect of seed treatment with carbamate and nitroguanidine insecticides to control thrips on beans. In: X Simpósio de Iniciação Científica, 2000, Viçosa, Brazil UFV, 2000. p. 219-219.

9. Crespo, A. L. B. ; Picanço, M. ; Galvan, T. L. ; Pereira, E. J. G. ; Simeão, A. A. Efficiency of insecticides for aphid control on collard. In: 40th Congresso Brasileiro De Olericultura, 2000, São Pedro. Poster presentation:Horticultura Brasileira, 2000. v. 18. p. 382-384.

10. Silva, Ézio Marques da ; Picanço, M. ; Crespo, A. L. B. ; Bacci, L. ; Antônio, Adilson de Castro. Efficacy of insecticides for thrips and leaf-miner control on cucumber. In: 40th Congresso Brasileiro De Olericultura, 2000, São Pedro. Poster presentation: Horticultura Brasileira, 2000. v. 18. p. 378-380.

11.Galvan, T. L. ; Picanço, M. ; Bacci, L. ; Crespo, A. L. B. ; Silva, F. M. Efficacy of five insecticides for thrips control on garlic. In: 40th Congresso Brasileiro De Olericultura, 2000, São Pedro. Poster presentation: Horticultura Brasileira, 2000. v. 18. p. 398-399.

Research Assistant Experience

Graduate Research Assistant, 2004 – 2007

Coordinator, facilitator and cooperator on projects at the Insect toxicology laboratory from UNL – United States.

Graduate Research Assistant, 2001 - 2003

Coordinator, facilitator and cooperator on projects at the Integrated Pest Management laboratory from UFV – Brazil.

Undergraduate Research Assistant, 1998 – 2001

Cooperator on projects at the Integrated Pest Management laboratory from UFV – Brazil.

Teaching Experience

-   Teaching assistant (August 2007 - present) for Entomology Department (UNL) Insect Identification 116 (undergraduate course).

-   Coordinator of the project “Enhancing Distance and Resident Education Delivery with Molting and Nerve Function Animations.” UNL's Department of Entomology (2006 – 2007).

-   Guest lecturer for Invertebrate Zoology 210 and Agricultural Entomology 360 (2003, undergraduate courses, UFV-Brazil).

-   Teaching assistant (Mar 1998 – July 1998) for Plant Biology Department, Plant Biology 210 (undergraduate course, UFV-Brazil).

Extension/Outreach Experience

-   Volunteer in the Open House “Experience the Power of Bugs” (2007) presented by UNL's Department of Entomology, Lincoln.

-   Elected Vice-president of the Brunner Club UNL student association (2007).

-   Volunteer in the Bug Bash event (2004, 2005, 2006) presented by UNL's Department of Entomology, Lincoln.

-   Volunteer in outreach events to train pest surveillance and pesticide application to growers of horticulture crops (2001, 2002, 2003).

Skills

Project management

-   Responsible by toxin production, insect selection, bioassays, greenhouse trials, and laboratory experiments (PhD).

-   Responsible by tomato growth and insect rearing to streamline insecticide bioassays assuring availability of biological material for large scale bioassays (Monitoring susceptibility of Tuta absoluta to insecticides, IRAC-BR, Insecticide Resistance Action Committee, Brazil).

Computer skills

-   Experience of PoloPlus, SAS, BIAevaluation, EndNote, Quantity One, and SigmaPlot.