Dr. Elane Fishilevich

fishilevich picture Adjunct Associate Professor
Principal Scientist
Alnylam Pharmaceuticals
300 Third Street, Cambridge, MA 02142

Email: efishilevich2@unl.edu
Website: http://www.alnylam.com/


Appointment: Adjunct Associate Professor

Education:

  1. B.A. in Biochemistry, Vassar College, 1999
  2. Ph.D. in Biomedical Sciences, The Rockefeller University, 2006
  3. Post doc in Biological Sciences, Carnegie Mellon University, 2010

Professional Responsibilities and Activities: ESA North Central branch Audit Committee.

Academic Interests: Cellular mechanisms of RNAi, including dsRNA and siRNA delivery in insects and vertebrates.

Publications:

  • Camargo, C., Wu, K., Fishilevich, E., Narva, K.E., and Siegfried, B.D. (2018). Knockdown of RNA interference pathway genes in western corn rootworm, Diabrotica virgifera virgifera, identifies no fitness costs associated with Argonaute 2 or Dicer-2. Pestic Biochem Physiol 148, 103-110.
  • Knorr, E., Fishilevich, E., Tenbusch, L., Frey, M.L.F., Rangasamy, M., Billion, A., Worden, S.E., Gandra, P., Arora, K., Lo, W., et al. (2018). Gene silencing in Tribolium castaneum as a tool for the targeted identification of candidate RNAi targets in crop pests. Sci Rep. 8(1):2061. doi: 10.1038/s41598-018-20416-y.
  • Bally, J., Fishilevich, E., Bowling, A.J., Pence, H.E., Narva, K.E., and Waterhouse, P.M. (2018) Next-Gen insect-proofing: expressing dsRNA in the Chloroplast. Pest Management Science, Jan 27. doi: 10.1002/ps.4870.
  • Wu, K., Camargo, C., Fishilevich, E., Narva, K.E., Chen, X., Taylor, C.E., and Siegfried, B.D. (2017). Distinct fitness costs associated with the knockdown of RNAi pathway genes in western corn rootworm adults. PLoS One, Dec 21;12(12):e0190208.
  • Vélez A. M., Fishilevich E., Matz N., Storer N. P., Narva K. E., and Siegfried B. D. (2016). Parameters for Successful Parental RNAi as An Insect Pest Management Tool in Western Corn Rootworm, Diabrotica virgifera virgifera,. Genes (Basel). Dec 24;8(1).
  • Shukla, J.N., Kalsi, M., Sethi, A., Narva, K.E., Fishilevich, E., Singh, S., Mogilicherla, K., and Palli, S.R. (2016). Reduced stability and intracellular transport of dsRNA contribute to poor RNAi response in lepidopteran insects. RNA Biol. Jul 2;13(7):656-69.
  • Fishilevich, E., Vélez A. M., Storer, N.P., Li, H., Bowling, A.J., Rangasamy, M., Worden, S.E., Narva, K.E., and Siegfried, B.D. (2016). RNAi as a management tool for the western corn rootworm, Diabrotica virgifera virgifera. Pest Manag Sci. Sep;72(9):1652-63.
  • Fishilevich, E., Vélez A. M., Khajuria, C., Frey, M. L. F., Ronda L. Hamm, Haichuan Wang, Greg A. Schulenberg, Andrew J. Bowling, Heather E. Pence, Premchand Gandra, Kanika Arora, Kenneth E. Narva, and Blair D. Siegfried. (2016). Use of Chromatin Remodeling ATPases as RNAi Targets for Multigenerational Control of Western Corn Rootworm (Diabrotica virgifera virgifera) and Neotropical Brown Stink Bug (Euschistus heros). Insect biochemistry and molecular biology 71, 58-71. [First description of RNAi in stink bugs, and identification of epigenetic gene family for multigenerational RNAi-based control of crop pests.]
  • Khajuria, C., Rangasamy, M., Vélez Arango, A. M., Wang, H., Fishilevich, E., Narva, K. E., and Siegfried, B. D. (2015). Parental RNA Interference of Genes Involved in Embryonic Development of the Western Corn Rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae). Insect Biochemistry and Molecular Biology. 63, 54-62. [Featured on GenomeWeb; first description of multigenerational RNAi-based control in a crop pest.]
  • Kim, S. W., Fishilevich, E., Arango-Argoty, G., Lin, Y., Liu, G., Li, Z., Watkins, S. C., Ahrendt, G., Monaghan, A. P., Nichols, M. D. and John, B. (2015). Genome-wide transcript profiling reveals novel breast cancer-associated intronic sense RNAs. PLoS One. Mar 23;10(3):e0120296 [Identifies novel breast cancer biomarkers using over 50 cancer patient samples and studies in mice using both high‐density arrays and deep‐sequencing.]
  • Ozsolak, F., Kapranov, P., Foissac, S., Kim, S.W., Fishilevich, E., Monaghan, A.P., John, B., and Milos, P.M. (2010). Comprehensive polyadenylation site maps in yeast and human reveal pervasive alternative polyadenylation. Cell 143, 1018-1029. [Genome‐wide analysis of polyadenylation sites in human RNAs and computational identification of novel sequence motifs.]
  • Fishilevich, E., Fitzpatrick, J. A., and Minden, J. S. pHMA, a pH-sensitive GFP reporter for cell engulfment in Drosophila embryos, tissues and cells. (2010). Dev. Dynamics, 239, 559-573. [A novel genetic reporter for phagocytosis of cells in animals and in cell culture.]
  • Fishilevich, E., Domingos, A. I., Asahina, K., Naef, F., Vosshall, L. B., and Louis, M. (2005). Chemotaxis behavior mediated by single larval olfactory neurons in Drosophila. Curr Biol 15, 2086-2096. [Featured in Current Biology Dispatch: 2006 Jan 10;16(1):R16-8; genetic ablation of specific olfactory neurons correlates with changes in behavior.]
  • Fishilevich, E., and Vosshall, L. B. (2005). Genetic and functional subdivision of the Drosophila antennal lobe. Curr Biol 15, 1548-1553. [Featured in Current Biology Dispatch 2005 Sep 6;15(17):R668-70; a near-complete receptor-based map to olfactory neurons in the brain.]
  • Hummel, T., Vasconcelos, M. L., Clemens, J. C., Fishilevich, E., Vosshall, L. B., and Zipursky, S. L. (2003). Axonal targeting of olfactory receptor neurons in Drosophila is controlled by Dscam. Neuron 37, 221-231. [Different isoforms of Down Syndrome Cell Adhesion Molecule (Dscam) protein guide olfactory neurons in the brain.]
    * equal contribution

Published Patent Applications and Granted Patents:

  1. SIEGFRIED, B., NARVA K. E., ARORA, K., WORDEN S. E., KHAJURIA, C., FISHILEVICH, E., STORER N. P., FREY, M., HAMM, R. & VELEZ, A. M. 2016. Parental RNAi suppression of chromatin remodeling genes to control coleopteran pests. US patent US9994844B2.
  2. SIEGFRIED, B., NARVA K. E., ARORA, K., WORDEN S. E., KHAJURIA, C., FISHILEVICH, E., STORER N. P., FREY, M., HAMM, R. & VELEZ, A. M. 2016. Parental RNAi suppression of chromatin remodeling genes to control hemipteran pests. AR patent application AR2015104096A. TW patent application TW20154142140A. UY patent application UY201536438A.
  3. SIEGFRIED, B., NARVA K. E., ARORA, K., WORDEN S. E., KHAJURIA, C., FISHILEVICH, E., STORER N. P., FREY, M., HAMM, R. & VELEZ, A. M. 2016. Parental RNAi suppression of hunchback gene to control coleopteran pests. AR patent application AR2015104098A. TW patent application TW20154142141A. UY patent application UY201536440A.
  4. SIEGFRIED, B., NARVA K. E., ARORA, K., WORDEN S. E., KHAJURIA, C., FISHILEVICH, E., STORER N. P., FREY, M., HAMM, R. & VELEZ, A. M. 2016. Parental RNAi suppression of hunchback gene to control hemipteran pests. AR patent application AR2015104099A. TW patent application TW20154142143A. UY patent application UY201536441A.
  5. SIEGFRIED, B., NARVA K. E., ARORA, K., WORDEN S. E., KHAJURIA, C., FISHILEVICH, E., STORER N. P., FREY, M., HAMM, R. & VELEZ, A. N. A. 2016d. Parental RNAi suppression of KRUPEL GENE to control coleopteran pests. AR patent application AR2015104100A. TW patent application TW20154142144A. UY patent application UY201536435A.
  6. SIEGFRIED B. D., NARVA K. E., ARORA K., WORDEN S. E., KHAJURIA C., FISHILEVICH E., STORER N. P., FREY M., HAMM R. L., & VELEZ A. M. 2016. PARENTAL RNAI SUPPRESSION OF KRUPPEL GENE TO CONTROL HEMIPTERAN PESTS. AR patent application AR2015104101A. US patent application US14971550A. UY patent application UY201536442A.
  7. NARVA, K. E., LI, H., GENG, C., ELANGO, N., HENRY M. J., RANGASAMY, M., WOOSLEY A. T., ARORA, K., GANDRA, P., WORDEN S. E. & FISHILEVICH, E. 2017. COPI coatomer alpha subunit nucleic acid molecules that confer resistance to coleopteran and hemipteran pests. AR patent application AR2015103303A. AU patent application AU2015333922A. UY patent application UY201536352A.
  8. NARVA, K E., LI, H., GENG, C., ELANGO, N., HENRY M. J., RANGASAMY, M., WOOSLEY A. T., ARORA, K., GANDRA, P., WORDEN S. E. & FISHILEVICH, E. 2017. COPI coatomer beta subunit nucleic acid molecules that confer resistance to coleopteran and hemipteran pests. AU patent application AU2015333923A. UY patent application UY201536359A.
  9. NARVA, K. E., LI, H., GENG, C., ELANGO, N., HENRY M. J., RANGASAMY, M., WOOSLEY A. T., ARORA, K., GANDRA, P., WORDEN S. E. & FISHILEVICH, E. 2017. COPI coatomer gamma subunit nucleic acid molecules that confer resistance to coleopteran and hemipteran pests. AU patent application AU2015333921A. UY patent application UY201536353A.
  10. NARVA, K. E., LI, H., RANGASAMY, M., ARORA, K., GANDRA, P., WORDEN S, E. & FISHILEVICH, E. 2017. COPI coatomer delta subunit nucleic acid molecules that confer resistance to coleopteran and hemipteran pests. AU patent application AU2015333924A. UY patent application UY201536358A.
  11. NARVA K. E., WORDEN S, E., FREY M. L. F., RANGASAMY, M., GANDRA, P., LO, W., FISHILEVICH, E., VILCINSKAS, A. & KNORR, E. 2017. PRP8 nucleic acid molecules to control insect pests. TW patent application TW20165122425A. US patent application US15394576A.
  12. NARVA K. E., FISHILEVICH E., RANGASAMY M., FREY MEGHAN L., LO W., WORDEN S. E., & GANDRA P. 2017. RAB5 NUCLEIC ACID MOLECULES THAT CONFER RESISTANCE TO COLEOPTERAN AND HEMIPTERAN PESTS. WO patent application WO2016US59248A.
  13. NARVA K. E., WORDEN SARAH E., FREY MEGHAN L., RANGASAMY M., GANDRA P., VEERAMANI B., LO W., FISHILEVICH E., VILCINSKAS A. & KNORR E. 2016. RNA POLYMERASE I1 NUCLEIC ACID MOLECULES TO CONTROL INSECT PESTS. US patent application US15069613A.
  14. NARVA K. E., WORDEN S., FREY M., RANGASAMY M., GANDRA P., VEERAMANI B., LO W., FISHILEVICH E., VILCINSKAS A. & KNORR E. 2016. RNA POLYMERASE II33 NUCLEIC ACID MOLECULES TO CONTROL INSECT PESTS. US patent application US15069689A. UY patent application UY201636582A.
  15. NARVA, K. E., WORDEN S. E., FREY M. L., RANGASAMY, M., GANDRA, P., VEERAMANI, B., LO, W., VILCINSKAS, A., KNORR, E. & FISHILEVICH, E. 2016. RNA polymerase II215 nucleic acid molecules to control insect pests. TW patent application TW20165107787A. UY patent application UY201636583A.
  16. NARVA K. E., LI, H., GENG, C., ARORA, K., VEERAMANI, B., GANDRA, P., WORDEN S. E., VILCINSKAS, A., KNORR, E., FISHILEVICH, E., RANGASAMY, M. & FREY MEGHAN, L. F. 2016. RAS OPPOSITE (ROP) AND RELATED NUCLEIC ACID MOLECULES THAT CONFER RESISTANCE TO COLEOPTERAN AND HEMIPTERAN PESTS. AP patent application AP20169318A. IL patent application IL246283A. JP patent application JP2016541168A. UY patent application UY201435912A.
  17. NARVA K. E., ARORA, K., WORDEN S. E., RANGASAMY, M., LI, H., SIEGFRIED, B., HKAJURIA, C. & FISHILEVICH, E. 2016. SEC23 NUCLEIC ACID MOLECULES THAT CONFER RESISTANCE TO COLEOPTERAN AND HEMIPTERAN PESTS. AP patent application AP20169558A. EP patent application EP2015789290A. IL patent application IL248738A. UY patent application UY201536114A.
  18. NARVA K. E., ARORA K., WORDEN S., RANGASAMY M., LI H., FREY M., SIEGFRIED B., KHAJURIA C., & FISHILEVICH E. 2017. GHO/SEC24B2 AND SEC24B1 NUCLEIC ACID MOLECULES TO CONTROL COLEOPTERAN AND HEMIPTERAN PESTS. UY patent application UY201536349A. WO patent application WO2015US54749A.
  19. NARVA K. E., WORDEN S., FREY M. L. F., RANGASAMY M., ARORA K., VEERAMANI B., GANDRA P., FISHILEVICH E., GENG C., VILCINSKAS A. & KNORR E. 2017. SHIBIRE/DYNAMIN NUCLEIC ACID MOLECULES TO CONTROL COLEOPTERAN AND HEMIPTERAN PESTS. US patent application US15276598A.
  20. NARVA K. E., WORDEN S., FREY M., RANGASAMY M., GANDRA P., LO W., FISHILEVICH E., VILCINSKAS A. & KNORR E. 2017. SNAP25 NUCLEIC ACID MOLECULES TO CONTROL INSECT PESTS. US patent application US15208039A.
  21. NARVA K. E., WORDEN S. E., FREY M., GANDRA P., LO W., FISHILEVICH E., RANGASAMY M., GENG C., VILCINSKAS A. & KNORR E. 2016. SPT5 NUCLEIC ACID MOLECULES TO CONTROL INSECT PESTS. UY patent application UY201636691A. WO patent application WO2016US34524A.
  22. NARVA, K. E., WORDEN S. E., FREY M. L., RANGASAMY, M., GANDRA, P., LO, W., FISHILEVICH, E., VILCINSKAS, A. & KNORR, E. 2017. SPT6 nucleic acid molecules to control insect pests. TW patent application TW20165116694A. UY patent application UY201636690A.
  23. NARVA, K. E, FISHILEVICH, E., FREY M. L. F., RANGASAMY, M., WORDEN, S. & GANDRA, P. 2017. THREAD nucleic acid molecules that confer resistance to hemipteran pests. TW patent application TW20165116304A. UY patent application UY201636685A.
  24. NARVA K. E., FISHILEVICH E., RANGASAMY M., FREY M. L., LO W., WORDEN S. E. & GANDRA P. 2017. WUPA NUCLEIC ACID MOLECULES THAT CONFER RESISTANCE TO COLEOPTERAN AND HEMIPTERAN PESTS. WO patent application WO2016US55300A.
  25. NARVA K. E., FISHILEVICH, E., RANGASAMY, M., FREY, M. L. LO, W., WORDEN S. E., GANDRA P. RIBOSOMAL PROTEIN L40 (RPL40) NUCLEIC ACID MOLECULES THAT CONFER RESISTANCE TO COLEOPTERAN AND HEMIPTERAN PESTS. Application number: US15376771
  26. NARVA K. E., WORDEN S. E, FREY M. L., RANGASAMY M., GANDRA P., LO W., FISHILEVICH, E., FISCHER R., VILCINSKAS A., KNORR E. GAWKY (GW) NUCLEIC ACID MOLECULES TO CONTROL INSECT PESTS. Application number: US15421233
  27. NARVA K. E., WORDEN S. E, FREY M. L., RANGASAMY, M., GANDRA P., LO W., FISHILEVICH E., FISCHER R., VILCINSKAS A., KNORR E. RPB7 NUCLEIC ACID MOLECULES TO CONTROL INSECT PESTS. Application number: US15421217
  28. NARVA K. E., WORDEN S. E, FREY M. L., RANGASAMY, M., GANDRA P., LO W., FISHILEVICH E., FISCHER R., VILCINSKAS A., KNORR E. FSH NUCLEIC ACID MOLECULES TO CONTROL INSECT PESTS. PUBLICATION NUMBER: 20170283827. U.S. PROVISIONAL PATENT APPLICATION SER. NO. 62/318,621.